A conserved and regulated mechanism drives endosomal Rab transition.

Endosomes and lysosomes harbor Rab5 and Rab7 on their surface as key proteins involved in their identity, biogenesis, and fusion. Rab activation requires a guanine nucleotide exchange factor (GEF), which is Mon1-Ccz1 for Rab7. During endosome maturation, Rab5 is replaced by Rab7, though the underlying mechanism remains poorly understood. Here, we identify the molecular determinants for Rab conversion in vivo and in vitro, and reconstitute Rab7 activation with yeast and metazoan proteins.

We show (i) that Mon1-Ccz1 is an effector of Rab5, (ii) that membrane-bound Rab5 is the key factor to directly promote Mon1-Ccz1 dependent Rab7 activation and Rab7-dependent membrane fusion, and (iii) that this process is regulated in yeast by the casein kinase Yck3, which phosphorylates Mon1 and blocks Rab5 binding.

AlbuVoid Buffer Kit - 500

AVBK-500 500 mL
EUR 615
Description: Albuvoid™ Buffer

HemoVoid Buffer Kit - 500

HVBK-500 500 mL
EUR 615
Description: HemoVoid™ Buffer

500 BlotBot Reagent Reservoirs

RRVR-500 500pack
EUR 534
Description: BlotBot reagent reservoirs will allow dispensing of unique reagents tray during processing. Pack of 500.

UPCK Buffer Kit - 500

UPBK-500 500 mL
EUR 615
Description: UPCK™ Buffer

WASH BOTTLE 500 ML, LDPE

1300P-500 12/pk
EUR 81
Description: Reusable Plastics; Resusable Bottles

REAGENT BOTTLE,500 ML, PP

1500P-500 10/pk
EUR 62
Description: Reusable Plastics; Resusable Bottles

ERLENMEYER FLASK, 500 ML, PP

4985P-500 6/pk
EUR 64
Description: Reusable Plastics; Reusable Erl Flasks

ERLENMEYER FLASK, 500 ML, PMP

4990P-500 6/pk
EUR 92
Description: Reusable Plastics; Reusable Erl Flasks

DBCO-PEG-Biotin,500

HE048041-500-100mg 100mg
EUR 765
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

PLGA(50:50)?8K?-PEG-Mal,500

HE086022-500-500mg 500mg
EUR 765
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Alkyne CPG modifier 500, 500 mg

32860 500 mg
EUR 193

FAM CPG 500, 6-isomer, 500 mg

35760 500 mg
EUR 249

TFA-Amino modifier CPG 500, 500 mg

31860 500 mg
EUR 193

miRZip-500 anti-miR-500 microRNA construct

MZIP500-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Cellufine S-500

21-200 100 ml
EUR 388

Cellufine S-500

21-201 500 ml
EUR 697

Cellufine S-500

21-202 5 lt Ask for price

Cellufine S-500

21-203 10 lt Ask for price

Cellufine A-500

675980327 100 ml
EUR 324

Cellufine A-500

675980335 10 lt Ask for price

Cellufine Q-500

675982327 100 ml
EUR 366

Cellufine Q-500

675982335 10 lt Ask for price

Cellufine C-500

675983327 100 ml
EUR 324

Cellufine C-500

675983335 10 lt Ask for price

Cellufine A-500

19805 500 ml
EUR 526

Cellufine A-500

19806 5 lt Ask for price

Cellufine C-500

19865 500 ml
EUR 526

Cellufine C-500

19866 5 lt Ask for price

Cellufine Q-500

19907 500 ml
EUR 718

Cellufine Q-500

19908 5 lt Ask for price

ZeptoGel (500 mL)

0801195 500 mL
EUR 327.68
  • What is the product classification?
  • ZeptoGel is marked as RUO.
Description: Please contact Gentaur in order to receive the datasheet of the product.

IMDM, 500 ml

411 500 ml
EUR 89

mPEG-SH,500

33-MF001003-500
  • EUR 259.00
  • EUR 677.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

mPEG-COOH?500

33-MF001017-500
  • EUR 198.00
  • EUR 494.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

mPEG-MAL,500

33-MF001022-500
  • EUR 235.00
  • EUR 605.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Measuring Spoon 500

MSP05 25pack
EUR 123
Description: 500uL (0.5mL) measuring spoon. Ideal for aliquoting beads into tubes.

500 ML SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS

4500-500 1/pk
EUR 529
Description: Bioprocess Vessels; Spinner Flask 70

TUBE,MICENT,1.7ML,NAT,SC,NS,BK,500/500

3620 500/pk
EUR 56
Description: Microcentrifuge Tubes; Snap Cap Tubes

Cal-500™ AM

20412 10x50 ug
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

MC Cellufine S-500

21-200-51 5 x 1 ml
EUR 239

MC Cellufine S-500

21-200-55 5 x 5 ml
EUR 420

Protonex™ Green 500

21215 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

PLASTIC CLAMP, 500 ML

480151 1/pk
EUR 70
Description: Lab Equipment; Constant Temperature Equipment

MC Cellufine C-500

19-800-51 5 x 1 ml
EUR 217

MC Cellufine C-500

19-800-55 5 x 5 ml
EUR 345

MC Cellufine A-500

19-805-51 5 x 1 ml
EUR 217

MC Cellufine A-500

19-805-55 5 x 5 ml
EUR 345

MC Cellufine Q-500

19-907-51 5 x 1 ml
EUR 217

MC Cellufine Q-500

19-907-55 5 x 5 ml
EUR 345

Augmedium™, Powder 500

124 500 ml
EUR 566

500 G HEPES, POWDER

61-034-RO 500 g/pk
EUR 146
Description: Media Catalog; Cell Culture Reagents

Refolding Buffers - 500 ml

612 500 ml
EUR 247

BMZERO™, 500 ml

401 500 ml
EUR 105

EPM2™, 500 ml

402 500 ml
EUR 107

HPC1™, 500 ml

403 500 ml
EUR 110

cleaning solution 500 ml

B530 ea
EUR 30

Biotin-PEG-COOH,500

33-HE041017-500
  • EUR 605.00
  • EUR 1714.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SH-PEG-SH,500

33-HO003003-500
  • EUR 259.00
  • EUR 677.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

AlKyne-PEG-AlKyne,500

33-HO007007-500
  • EUR 283.00
  • EUR 747.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

COOH-PEG-COOH,500

33-HO017017-500
  • EUR 167.00
  • EUR 399.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SC-PEG-SC,500

33-HO023023-500
  • EUR 167.00
  • EUR 399.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

THPTA ligand, 500 mg

H4050 500 mg
EUR 216

HO-PEG-COOH,500

33-HE002017-500
  • EUR 198.00
  • EUR 494.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SH-PEG-COOH,500

33-HE003017-500
  • EUR 519.00
  • EUR 1456.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

HS-PEG-NHS,500

33-HE003023-500
  • EUR 445.00
  • EUR 1234.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

NH2-PEG-COOH,500

33-HE005017-500
  • EUR 359.00
  • EUR 975.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

N3-PEG-OH,500

33-HE006002-500
  • EUR 568.00
  • EUR 1603.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

N3-PEG-BIOTIN,500

33-HE006041-500
  • EUR 827.00
  • EUR 2380.00
  • 1g
  • 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Silicone oil, 500 cSt

GK1916-1L 1 l
EUR 150

Silicone oil, 500 cSt

GK1916-250ML 250 ml
EUR 78

rno-miR-500 Primers

MP-r00247 150 ul / 10 uM
EUR 176

mmu-miR-500 Primers

MPM00559 150 ul / 10 uM
EUR 121

Hinc II unit: 500

YRHINC2 1 vial Ask for price

Nru I unit: 500

YRNRU1 1 vial Ask for price

Cal-500™, potassium salt

20410 10x50 ug
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Protonex™ Green 500, SE

21216 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Protonex™ Green 500 Dextran

21217 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Long trebler phosphoramidite, 500 mg

34260 500 mg
EUR 216

Alkyne CPG 1000A, 500 mg

35860 500 mg
EUR 193

T4 DNA Polymerase - 500 units

3222 1/EA
EUR 206

mFluor™ Violet 500 SE

1149 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

APF LB Lennox, 500 g

132 500 g
EUR 107

APF LB Miller, 500 g

133 500 g
EUR 105

500 ML MCDB 131, 1X

15-100-CV 500 mL/pk
EUR 132
Description: Media Catalog; Classical Media

LB*Booster™, Powder 500

126 500 ml
EUR 190

500 G L-GLUTAMINE, POWDER

61-030-RO 500 g/pk
EUR 199
Description: Media Catalog; Cell Culture Reagents

500 G POLYSUCROSE 400, POWDER

61-196-RO 500 g/pk
EUR 507
Description: Specialty Media; Insect Media Products

500 G YEAST EXTRACT, POWDER

61-231-RO 500 g/pk
EUR 178
Description: Media Catalog; Cell Culture Reagents

500 ML SODIUM CHLORIDE, 5M

46-032-CV 500 mL/pk
EUR 90
Description: Media Catalog; Classical Media

500 ML HYBRIGRO SF™

40-215-CVR 500 mL/pk
EUR 200
Description: Specialty Media; Serum Free and Specialty Media

P4-8F™, 500 ml

404 500 ml
EUR 107

DiagNano Silica Particles, 500 nm

DNG-B009 10 mL
EUR 559

Polyclonal CDC6 Antibody (aa400-500)

APR02453G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CDC6 (aa400-500). This antibody is tested and proven to work in the following applications:

Polyclonal ZBTB2 Antibody (aa450-500)

APR02982G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ZBTB2 (aa450-500). This antibody is tested and proven to work in the following applications:

electrolyte 500 ml 4m licl

B525 ea
EUR 121

BioRad CFX96, Megabase 500/1000

A28241L 5x25 pcs
EUR 303

SharpProtein MarkerII unit: 500 µl

FYP005-500UL 1 vial Ask for price

mmu-miR-500-3p Primers

MPM01366 150 ul / 10 uM
EUR 121

Our study thus uncovers the minimal feed-forward machinery of the endosomal Rab cascade and a novel regulatory mechanism controlling this pathway.