Endosomes and lysosomes harbor Rab5 and Rab7 on their surface as key proteins involved in their identity, biogenesis, and fusion. Rab activation requires a guanine nucleotide exchange factor (GEF), which is Mon1-Ccz1 for Rab7. During endosome maturation, Rab5 is replaced by Rab7, though the underlying mechanism remains poorly understood. Here, we identify the molecular determinants for Rab conversion in vivo and in vitro, and reconstitute Rab7 activation with yeast and metazoan proteins.
We show (i) that Mon1-Ccz1 is an effector of Rab5, (ii) that membrane-bound Rab5 is the key factor to directly promote Mon1-Ccz1 dependent Rab7 activation and Rab7-dependent membrane fusion, and (iii) that this process is regulated in yeast by the casein kinase Yck3, which phosphorylates Mon1 and blocks Rab5 binding.
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HVBK-500 | Biotech Support Group | 500 mL | EUR 615 |
Description: HemoVoid™ Buffer |
![]() UPCK Buffer Kit - 500 |
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UPBK-500 | Biotech Support Group | 500 mL | EUR 615 |
Description: UPCK™ Buffer |
![]() ERLENMEYER FLASK, 500 ML, PP |
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4985P-500 | CORNING | 6/pk | EUR 64 |
Description: Reusable Plastics; Reusable Erl Flasks |
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4990P-500 | CORNING | 6/pk | EUR 92 |
Description: Reusable Plastics; Reusable Erl Flasks |
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1300P-500 | CORNING | 12/pk | EUR 81 |
Description: Reusable Plastics; Resusable Bottles |
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1500P-500 | CORNING | 10/pk | EUR 62 |
Description: Reusable Plastics; Resusable Bottles |
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HE048041-500-100mg | Biochempeg | 100mg | EUR 765 |
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32860 | lumiprobe | 500 mg | EUR 193 |
![]() PLGA(50:50)?8K?-PEG-Mal,500 |
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HE086022-500-500mg | Biochempeg | 500mg | EUR 765 |
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35760 | lumiprobe | 500 mg | EUR 249 |
![]() TFA-Amino modifier CPG 500, 500 mg |
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31860 | lumiprobe | 500 mg | EUR 193 |
![]() miRZip-500 anti-miR-500 microRNA construct |
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MZIP500-PA-1 | SBI | Bacterial Streak | EUR 684 |
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411 | AthenaES | 500 ml | EUR 89 |
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MSP05 | Next Advance | 25pack | EUR 123 |
Description: 500uL (0.5mL) measuring spoon. Ideal for aliquoting beads into tubes. |
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0801195 | Zeptometrix | 500 mL | EUR 327.68 |
Description: Please contact Gentaur in order to receive the datasheet of the product. |
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19805 | JNC America | 500 ml | EUR 526 |
![]() Cellufine A-500 |
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19806 | JNC America | 5 lt | Ask for price |
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19865 | JNC America | 500 ml | EUR 526 |
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19866 | JNC America | 5 lt | Ask for price |
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19907 | JNC America | 500 ml | EUR 718 |
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21-200 | JNC America | 100 ml | EUR 388 |
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21-201 | JNC America | 500 ml | EUR 697 |
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21-202 | JNC America | 5 lt | Ask for price |
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21-203 | JNC America | 10 lt | Ask for price |
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675980327 | JNC America | 100 ml | EUR 324 |
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675980335 | JNC America | 10 lt | Ask for price |
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675982327 | JNC America | 100 ml | EUR 366 |
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675982335 | JNC America | 10 lt | Ask for price |
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675983327 | JNC America | 100 ml | EUR 324 |
![]() Cellufine C-500 |
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675983335 | JNC America | 10 lt | Ask for price |
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33-MF001003-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-MF001017-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-MF001022-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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3620 | CORNING | 500/pk | EUR 56 |
Description: Microcentrifuge Tubes; Snap Cap Tubes |
![]() 500 ML SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS |
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4500-500 | CORNING | 1/pk | EUR 529 |
Description: Bioprocess Vessels; Spinner Flask 70 |
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H4050 | lumiprobe | 500 mg | EUR 216 |
![]() Refolding Buffers - 500 ml |
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612 | AthenaES | 500 ml | EUR 247 |
![]() EPM2™, 500 ml |
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402 | AthenaES | 500 ml | EUR 107 |
![]() Cal-500™ AM |
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20412 | AAT Bioquest | 10x50 ug | EUR 219 |
![]() Protonex™ Green 500 |
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21215 | AAT Bioquest | 1 mg | EUR 219 |
![]() rno-miR-500 Primers |
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MP-r00247 | ABM | 150 ul / 10 uM | EUR 176 |
![]() mmu-miR-500 Primers |
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MPM00559 | ABM | 150 ul / 10 uM | EUR 121 |
![]() cleaning solution 500 ml |
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B530 | Consort | ea | EUR 30 |
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61-034-RO | CORNING | 500 g/pk | EUR 146 |
Description: Media Catalog; Cell Culture Reagents |
![]() PLASTIC CLAMP, 500 ML |
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480151 | CORNING | 1/pk | EUR 70 |
Description: Lab Equipment; Constant Temperature Equipment |
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GK1916-1L | Glentham Life Sciences | 1 l | EUR 150 |
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GK1916-250ML | Glentham Life Sciences | 250 ml | EUR 78 |
![]() MC Cellufine C-500 |
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19-800-51 | JNC America | 5 x 1 ml | EUR 217 |
![]() MC Cellufine C-500 |
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19-800-55 | JNC America | 5 x 5 ml | EUR 345 |
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19-805-51 | JNC America | 5 x 1 ml | EUR 217 |
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19-805-55 | JNC America | 5 x 5 ml | EUR 345 |
![]() MC Cellufine Q-500 |
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19-907-51 | JNC America | 5 x 1 ml | EUR 217 |
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19-907-55 | JNC America | 5 x 5 ml | EUR 345 |
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21-200-51 | JNC America | 5 x 1 ml | EUR 239 |
![]() MC Cellufine S-500 |
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21-200-55 | JNC America | 5 x 5 ml | EUR 420 |
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YRHINC2 | Yeastern Biotech | 1 vial | Ask for price |
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33-HE002017-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-HE003017-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-HE003023-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-HE005017-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-HE006002-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-HE006041-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-HE041017-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-HO003003-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-HO007007-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-HO017017-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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33-HO023023-500 | Biochempeg |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
![]() Long trebler phosphoramidite, 500 mg |
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34260 | lumiprobe | 500 mg | EUR 216 |
![]() Alkyne CPG 1000A, 500 mg |
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35860 | lumiprobe | 500 mg | EUR 193 |
![]() mFluor™ Violet 500 SE |
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1149 | AAT Bioquest | 1 mg | EUR 219 |
![]() Cal-500™, potassium salt |
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20410 | AAT Bioquest | 10x50 ug | EUR 219 |
![]() Protonex™ Green 500, SE |
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21216 | AAT Bioquest | 1 mg | EUR 219 |
![]() Protonex™ Green 500 Dextran |
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21217 | AAT Bioquest | 1 mg | EUR 219 |
![]() mmu-miR-500-3p Primers |
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MPM01366 | ABM | 150 ul / 10 uM | EUR 121 |
![]() mmu-miR-500-5p Primers |
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MPM01367 | ABM | 150 ul / 10 uM | EUR 121 |
![]() rno-miR-500-3p Primers |
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MPR00748 | ABM | 150 ul / 10 uM | EUR 176 |
![]() rno-miR-500-5p Primers |
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MPR00749 | ABM | 150 ul / 10 uM | EUR 176 |
![]() rno??miR-500 miRNA Antagomir |
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MNR01672 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-500 miRNA Antagomir |
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MNM02203 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-500 miRNA Inhibitor |
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MIM02203 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() rno??miR-500 miRNA Inhibitor |
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MIR01672 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() electrolyte 500 ml 4m licl |
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B525 | Consort | ea | EUR 121 |
![]() Compact Balance, 500 grams, 115V |
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BCM1302 | Bio Basic | 1 pcs, 1 UNIT | EUR 456.19 |
![]() Precision Balance, 500 grams, 115V |
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BCM1308 | Bio Basic | 1 pcs, 1 UNIT | EUR 1479.17 |
![]() Measuring Spoon 500 RNase Free |
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MSP05-RNA | Next Advance | 10pack | EUR 136 |
Description: 500uL (0.5mL) measuring spoon. Ideal for aliquoting beads into tubes. RNase free. |
![]() DiagNano Silica Particles, 500 nm |
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DNG-B009 | Creative Diagnostics | 10 mL | EUR 559 |
![]() T4 DNA Polymerase - 500 units |
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3222 | Intact Genomics | 1/EA | EUR 206 |
![]() 500 ML HYBRIGRO SF™ |
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40-215-CVR | CORNING | 500 mL/pk | EUR 200 |
Description: Specialty Media; Serum Free and Specialty Media |
![]() 500 G L-GLUTAMINE, POWDER |
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61-030-RO | CORNING | 500 g/pk | EUR 199 |
Description: Media Catalog; Cell Culture Reagents |
![]() 500 G POLYSUCROSE 400, POWDER |
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61-196-RO | CORNING | 500 g/pk | EUR 507 |
Description: Specialty Media; Insect Media Products |
![]() 500 G YEAST EXTRACT, POWDER |
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61-231-RO | CORNING | 500 g/pk | EUR 178 |
Description: Media Catalog; Cell Culture Reagents |
![]() BioRad CFX96, Megabase 500/1000 |
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A28241L | GeneOn | 5x25 pcs | EUR 303 |
![]() 500 ML SODIUM CHLORIDE, 5M |
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46-032-CV | CORNING | 500 mL/pk | EUR 90 |
Description: Media Catalog; Classical Media |
![]() 500 ML MCDB 131, 1X |
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15-100-CV | CORNING | 500 mL/pk | EUR 132 |
Description: Media Catalog; Classical Media |
Our study thus uncovers the minimal feed-forward machinery of the endosomal Rab cascade and a novel regulatory mechanism controlling this pathway.