Extracellular signal-regulated kinases

Endosomes and lysosomes harbor Rab5 and Rab7 on their surface as key proteins involved in their identity, biogenesis, and fusion. Rab activation requires a guanine nucleotide exchange factor (GEF), which is Mon1-Ccz1 for Rab7. During endosome maturation, Rab5 is replaced by Rab7, though the underlying mechanism remains poorly understood. Here, we identify the molecular determinants for Rab conversion in vivo and in vitro, and reconstitute Rab7 activation with yeast and metazoan proteins.

We show (i) that Mon1-Ccz1 is an effector of Rab5, (ii) that membrane-bound Rab5 is the key factor to directly promote Mon1-Ccz1 dependent Rab7 activation and Rab7-dependent membrane fusion, and (iii) that this process is regulated in yeast by the casein kinase Yck3, which phosphorylates Mon1 and blocks Rab5 binding.

HemoVoid Buffer Kit - 500
HVBK-500	Biotech Support Group	500 mL	EUR 615
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UPCK Buffer Kit - 500
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ERLENMEYER FLASK, 500 ML, PP
4985P-500	CORNING	6/pk	EUR 64
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4990P-500	CORNING	6/pk	EUR 92
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1500P-500	CORNING	10/pk	EUR 62
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DBCO-PEG-Biotin,500
HE048041-500-100mg	Biochempeg	100mg	EUR 765
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Alkyne CPG modifier 500, 500 mg
32860	lumiprobe	500 mg	EUR 193

PLGA(50:50)?8K?-PEG-Mal,500
HE086022-500-500mg	Biochempeg	500mg	EUR 765
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FAM CPG 500, 6-isomer, 500 mg
35760	lumiprobe	500 mg	EUR 249

TFA-Amino modifier CPG 500, 500 mg
31860	lumiprobe	500 mg	EUR 193

miRZip-500 anti-miR-500 microRNA construct
MZIP500-PA-1	SBI	Bacterial Streak	EUR 684

IMDM, 500 ml
411	AthenaES	500 ml	EUR 89

Measuring Spoon 500
MSP05	Next Advance	25pack	EUR 123
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ZeptoGel (500 mL)
0801195	Zeptometrix	500 mL	EUR 327.68
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19805	JNC America	500 ml	EUR 526

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19865	JNC America	500 ml	EUR 526

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H4050	lumiprobe	500 mg	EUR 216

Refolding Buffers - 500 ml
612	AthenaES	500 ml	EUR 247

EPM2™, 500 ml
402	AthenaES	500 ml	EUR 107

Cal-500™ AM
20412	AAT Bioquest	10x50 ug	EUR 219

Protonex™ Green 500
21215	AAT Bioquest	1 mg	EUR 219

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B530	Consort	ea	EUR 30

500 G HEPES, POWDER
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480151	CORNING	1/pk	EUR 70
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19-800-51	JNC America	5 x 1 ml	EUR 217

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33-HE006002-500	Biochempeg	EUR 568.00 EUR 1603.00	1g 5g
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33-HO007007-500	Biochempeg	EUR 283.00 EUR 747.00	1g 5g
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Long trebler phosphoramidite, 500 mg
34260	lumiprobe	500 mg	EUR 216

Alkyne CPG 1000A, 500 mg
35860	lumiprobe	500 mg	EUR 193

mFluor™ Violet 500 SE
1149	AAT Bioquest	1 mg	EUR 219

Cal-500™, potassium salt
20410	AAT Bioquest	10x50 ug	EUR 219

Protonex™ Green 500, SE
21216	AAT Bioquest	1 mg	EUR 219

Protonex™ Green 500 Dextran
21217	AAT Bioquest	1 mg	EUR 219

mmu-miR-500-3p Primers
MPM01366	ABM	150 ul / 10 uM	EUR 121

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MPM01367	ABM	150 ul / 10 uM	EUR 121

rno-miR-500-3p Primers
MPR00748	ABM	150 ul / 10 uM	EUR 176

rno-miR-500-5p Primers
MPR00749	ABM	150 ul / 10 uM	EUR 176

rno??miR-500 miRNA Antagomir
MNR01672	ABM	2 x 5.0 nmol	EUR 329

mmu-miR-500 miRNA Antagomir
MNM02203	ABM	2 x 5.0 nmol	EUR 329

mmu-miR-500 miRNA Inhibitor
MIM02203	ABM	2 x 5.0 nmol	EUR 176

rno??miR-500 miRNA Inhibitor
MIR01672	ABM	2 x 5.0 nmol	EUR 176

electrolyte 500 ml 4m licl
B525	Consort	ea	EUR 121

Compact Balance, 500 grams, 115V
BCM1302	Bio Basic	1 pcs, 1 UNIT	EUR 456.19

Precision Balance, 500 grams, 115V
BCM1308	Bio Basic	1 pcs, 1 UNIT	EUR 1479.17

Measuring Spoon 500 RNase Free
MSP05-RNA	Next Advance	10pack	EUR 136
Description: 500uL (0.5mL) measuring spoon. Ideal for aliquoting beads into tubes. RNase free.

DiagNano Silica Particles, 500 nm
DNG-B009	Creative Diagnostics	10 mL	EUR 559

T4 DNA Polymerase - 500 units
3222	Intact Genomics	1/EA	EUR 206

500 ML HYBRIGRO SF™
40-215-CVR	CORNING	500 mL/pk	EUR 200
Description: Specialty Media; Serum Free and Specialty Media

500 G L-GLUTAMINE, POWDER
61-030-RO	CORNING	500 g/pk	EUR 199
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500 G POLYSUCROSE 400, POWDER
61-196-RO	CORNING	500 g/pk	EUR 507
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500 G YEAST EXTRACT, POWDER
61-231-RO	CORNING	500 g/pk	EUR 178
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BioRad CFX96, Megabase 500/1000
A28241L	GeneOn	5x25 pcs	EUR 303

500 ML SODIUM CHLORIDE, 5M
46-032-CV	CORNING	500 mL/pk	EUR 90
Description: Media Catalog; Classical Media

500 ML MCDB 131, 1X
15-100-CV	CORNING	500 mL/pk	EUR 132
Description: Media Catalog; Classical Media

Our study thus uncovers the minimal feed-forward machinery of the endosomal Rab cascade and a novel regulatory mechanism controlling this pathway.