Extracellular signal-regulated kinases

Endosomes and lysosomes harbor Rab5 and Rab7 on their surface as key proteins involved in their identity, biogenesis, and fusion. Rab activation requires a guanine nucleotide exchange factor (GEF), which is Mon1-Ccz1 for Rab7. During endosome maturation, Rab5 is replaced by Rab7, though the underlying mechanism remains poorly understood. Here, we identify the molecular determinants for Rab conversion in vivo and in vitro, and reconstitute Rab7 activation with yeast and metazoan proteins.

We show (i) that Mon1-Ccz1 is an effector of Rab5, (ii) that membrane-bound Rab5 is the key factor to directly promote Mon1-Ccz1 dependent Rab7 activation and Rab7-dependent membrane fusion, and (iii) that this process is regulated in yeast by the casein kinase Yck3, which phosphorylates Mon1 and blocks Rab5 binding.

AlbuVoid Buffer Kit - 500
AVBK-500	Biotech Support Group	500 mL	EUR 615
Description: Albuvoid™ Buffer

HemoVoid Buffer Kit - 500
HVBK-500	Biotech Support Group	500 mL	EUR 615
Description: HemoVoid™ Buffer

500 BlotBot Reagent Reservoirs
RRVR-500	Next Advance	500pack	EUR 534
Description: BlotBot reagent reservoirs will allow dispensing of unique reagents tray during processing. Pack of 500.

UPCK Buffer Kit - 500
UPBK-500	Biotech Support Group	500 mL	EUR 615
Description: UPCK™ Buffer

WASH BOTTLE 500 ML, LDPE
1300P-500	CORNING	12/pk	EUR 81
Description: Reusable Plastics; Resusable Bottles

REAGENT BOTTLE,500 ML, PP
1500P-500	CORNING	10/pk	EUR 62
Description: Reusable Plastics; Resusable Bottles

ERLENMEYER FLASK, 500 ML, PP
4985P-500	CORNING	6/pk	EUR 64
Description: Reusable Plastics; Reusable Erl Flasks

ERLENMEYER FLASK, 500 ML, PMP
4990P-500	CORNING	6/pk	EUR 92
Description: Reusable Plastics; Reusable Erl Flasks

DBCO-PEG-Biotin,500
HE048041-500-100mg	Biochempeg	100mg	EUR 765
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

PLGA(50:50)?8K?-PEG-Mal,500
HE086022-500-500mg	Biochempeg	500mg	EUR 765
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Alkyne CPG modifier 500, 500 mg
32860	lumiprobe	500 mg	EUR 193

FAM CPG 500, 6-isomer, 500 mg
35760	lumiprobe	500 mg	EUR 249

TFA-Amino modifier CPG 500, 500 mg
31860	lumiprobe	500 mg	EUR 193

miRZip-500 anti-miR-500 microRNA construct
MZIP500-PA-1	SBI	Bacterial Streak	EUR 684
Category: MicroRNA Tools

Cellufine S-500
21-200	JNC America	100 ml	EUR 388

Cellufine S-500
21-201	JNC America	500 ml	EUR 697

Cellufine S-500
21-202	JNC America	5 lt	Ask for price

Cellufine S-500
21-203	JNC America	10 lt	Ask for price

Cellufine A-500
675980327	JNC America	100 ml	EUR 324

Cellufine A-500
675980335	JNC America	10 lt	Ask for price

Cellufine Q-500
675982327	JNC America	100 ml	EUR 366

Cellufine Q-500
675982335	JNC America	10 lt	Ask for price

Cellufine C-500
675983327	JNC America	100 ml	EUR 324

Cellufine C-500
675983335	JNC America	10 lt	Ask for price

Cellufine A-500
19805	JNC America	500 ml	EUR 526

Cellufine A-500
19806	JNC America	5 lt	Ask for price

Cellufine C-500
19865	JNC America	500 ml	EUR 526

Cellufine C-500
19866	JNC America	5 lt	Ask for price

Cellufine Q-500
19907	JNC America	500 ml	EUR 718

Cellufine Q-500
19908	JNC America	5 lt	Ask for price

ZeptoGel (500 mL)
0801195	Zeptometrix	500 mL	EUR 327.68
What is the product classification? ZeptoGel is marked as RUO.
Description: Please contact Gentaur in order to receive the datasheet of the product.

IMDM, 500 ml
411	AthenaES	500 ml	EUR 89

mPEG-SH,500
33-MF001003-500	Biochempeg	EUR 259.00 EUR 677.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

mPEG-COOH?500
33-MF001017-500	Biochempeg	EUR 198.00 EUR 494.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

mPEG-MAL,500
33-MF001022-500	Biochempeg	EUR 235.00 EUR 605.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Measuring Spoon 500
MSP05	Next Advance	25pack	EUR 123
Description: 500uL (0.5mL) measuring spoon. Ideal for aliquoting beads into tubes.

500 ML SPINNER FLASK, 100 MM CENTRE NECK, 2 X 45 MM SIDEARMS
4500-500	CORNING	1/pk	EUR 529
Description: Bioprocess Vessels; Spinner Flask 70

TUBE,MICENT,1.7ML,NAT,SC,NS,BK,500/500
3620	CORNING	500/pk	EUR 56
Description: Microcentrifuge Tubes; Snap Cap Tubes

Cal-500™ AM
20412	AAT Bioquest	10x50 ug	EUR 219
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12352200

MC Cellufine S-500
21-200-51	JNC America	5 x 1 ml	EUR 239

MC Cellufine S-500
21-200-55	JNC America	5 x 5 ml	EUR 420

Protonex™ Green 500
21215	AAT Bioquest	1 mg	EUR 219
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12352200

PLASTIC CLAMP, 500 ML
480151	CORNING	1/pk	EUR 70
Description: Lab Equipment; Constant Temperature Equipment

MC Cellufine C-500
19-800-51	JNC America	5 x 1 ml	EUR 217

MC Cellufine C-500
19-800-55	JNC America	5 x 5 ml	EUR 345

MC Cellufine A-500
19-805-51	JNC America	5 x 1 ml	EUR 217

MC Cellufine A-500
19-805-55	JNC America	5 x 5 ml	EUR 345

MC Cellufine Q-500
19-907-51	JNC America	5 x 1 ml	EUR 217

MC Cellufine Q-500
19-907-55	JNC America	5 x 5 ml	EUR 345

Augmedium™, Powder 500
124	AthenaES	500 ml	EUR 566

500 G HEPES, POWDER
61-034-RO	CORNING	500 g/pk	EUR 146
Description: Media Catalog; Cell Culture Reagents

Refolding Buffers - 500 ml
612	AthenaES	500 ml	EUR 247

BMZERO™, 500 ml
401	AthenaES	500 ml	EUR 105

EPM2™, 500 ml
402	AthenaES	500 ml	EUR 107

HPC1™, 500 ml
403	AthenaES	500 ml	EUR 110

cleaning solution 500 ml
B530	Consort	ea	EUR 30

Biotin-PEG-COOH,500
33-HE041017-500	Biochempeg	EUR 605.00 EUR 1714.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SH-PEG-SH,500
33-HO003003-500	Biochempeg	EUR 259.00 EUR 677.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

AlKyne-PEG-AlKyne,500
33-HO007007-500	Biochempeg	EUR 283.00 EUR 747.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

COOH-PEG-COOH,500
33-HO017017-500	Biochempeg	EUR 167.00 EUR 399.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SC-PEG-SC,500
33-HO023023-500	Biochempeg	EUR 167.00 EUR 399.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

THPTA ligand, 500 mg
H4050	lumiprobe	500 mg	EUR 216

HO-PEG-COOH,500
33-HE002017-500	Biochempeg	EUR 198.00 EUR 494.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

SH-PEG-COOH,500
33-HE003017-500	Biochempeg	EUR 519.00 EUR 1456.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

HS-PEG-NHS,500
33-HE003023-500	Biochempeg	EUR 445.00 EUR 1234.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

NH2-PEG-COOH,500
33-HE005017-500	Biochempeg	EUR 359.00 EUR 975.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

N3-PEG-OH,500
33-HE006002-500	Biochempeg	EUR 568.00 EUR 1603.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

N3-PEG-BIOTIN,500
33-HE006041-500	Biochempeg	EUR 827.00 EUR 2380.00	1g 5g
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Silicone oil, 500 cSt
GK1916-1L	Glentham Life Sciences	1 l	EUR 150

Silicone oil, 500 cSt
GK1916-250ML	Glentham Life Sciences	250 ml	EUR 78

rno-miR-500 Primers
MP-r00247	ABM	150 ul / 10 uM	EUR 176

mmu-miR-500 Primers
MPM00559	ABM	150 ul / 10 uM	EUR 121

Hinc II unit: 500
YRHINC2	Yeastern Biotech	1 vial	Ask for price

Nru I unit: 500
YRNRU1	Yeastern Biotech	1 vial	Ask for price

Cal-500™, potassium salt
20410	AAT Bioquest	10x50 ug	EUR 219
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12352200

Protonex™ Green 500, SE
21216	AAT Bioquest	1 mg	EUR 219
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12352200

Protonex™ Green 500 Dextran
21217	AAT Bioquest	1 mg	EUR 219
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12352200

Long trebler phosphoramidite, 500 mg
34260	lumiprobe	500 mg	EUR 216

Alkyne CPG 1000A, 500 mg
35860	lumiprobe	500 mg	EUR 193

T4 DNA Polymerase - 500 units
3222	Intact Genomics	1/EA	EUR 206

mFluor™ Violet 500 SE
1149	AAT Bioquest	1 mg	EUR 219
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

APF LB Lennox, 500 g
132	AthenaES	500 g	EUR 107

APF LB Miller, 500 g
133	AthenaES	500 g	EUR 105

500 ML MCDB 131, 1X
15-100-CV	CORNING	500 mL/pk	EUR 132
Description: Media Catalog; Classical Media

LB*Booster™, Powder 500
126	AthenaES	500 ml	EUR 190

500 G L-GLUTAMINE, POWDER
61-030-RO	CORNING	500 g/pk	EUR 199
Description: Media Catalog; Cell Culture Reagents

500 G POLYSUCROSE 400, POWDER
61-196-RO	CORNING	500 g/pk	EUR 507
Description: Specialty Media; Insect Media Products

500 G YEAST EXTRACT, POWDER
61-231-RO	CORNING	500 g/pk	EUR 178
Description: Media Catalog; Cell Culture Reagents

500 ML SODIUM CHLORIDE, 5M
46-032-CV	CORNING	500 mL/pk	EUR 90
Description: Media Catalog; Classical Media

500 ML HYBRIGRO SF™
40-215-CVR	CORNING	500 mL/pk	EUR 200
Description: Specialty Media; Serum Free and Specialty Media

P4-8F™, 500 ml
404	AthenaES	500 ml	EUR 107

DiagNano Silica Particles, 500 nm
DNG-B009	Creative Diagnostics	10 mL	EUR 559

Polyclonal CDC6 Antibody (aa400-500)
APR02453G	Leading Biology	0.05ml	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CDC6 (aa400-500). This antibody is tested and proven to work in the following applications:

Polyclonal ZBTB2 Antibody (aa450-500)
APR02982G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ZBTB2 (aa450-500). This antibody is tested and proven to work in the following applications:

electrolyte 500 ml 4m licl
B525	Consort	ea	EUR 121

BioRad CFX96, Megabase 500/1000
A28241L	GeneOn	5x25 pcs	EUR 303

SharpProtein MarkerII unit: 500 µl
FYP005-500UL	Yeastern Biotech	1 vial	Ask for price

mmu-miR-500-3p Primers
MPM01366	ABM	150 ul / 10 uM	EUR 121

Our study thus uncovers the minimal feed-forward machinery of the endosomal Rab cascade and a novel regulatory mechanism controlling this pathway.